Overexpression of Na/Ca exchanger alters contractility and SR Ca content in adult rat myocytes
نویسندگان
چکیده
XUE-QIAN ZHANG,1 JIANLIANG SONG,1 LAWRENCE I. ROTHBLUM,1 MINGYUE LUN,1 XUJUN WANG,1 FAN DING,1 JEREMY DUNN,2 JONATHAN LYTTON,2 PAUL J. MCDERMOTT,3 AND JOSEPH Y. CHEUNG1 1Weis Center for Research, Geisinger Medical Center, Danville, Pennsylvania 17822; 2Department of Biochemistry and Molecular Biology, University of Calgary Health Sciences Center, Calgary, Alberta, Canada T2N 4N1; and 3Department of Medicine, Medical University of South Carolina, and the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, South Carolina 29403
منابع مشابه
Overexpression of phospholemman alters contractility and [Ca(2+)](i) transients in adult rat myocytes.
Previous studies showed increased phospholemman (PLM) mRNA after myocardial infarction (MI) in rats (Sehl PD, Tai JTN, Hillan KJ, Brown LA, Goddard A, Yang R, Jin H, and Lowe DG. Circulation 101: 1990-1999, 2000). We tested the hypothesis that, in normal adult rat cardiac myocytes, PLM overexpression alters contractile function and cytosolic Ca(2+) concentration ([Ca(2+)](i)) homeostasis in a m...
متن کاملInduced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.
We have produced mice in which expression of the rat cardiac Na(+)/Ca(2+) exchanger (NCX1) transgene was switched on when doxycycline was removed from the feed at 5 wk. At 8 to 10 wk, NCX1 expression in induced (Ind) mouse hearts was 2.5-fold higher but protein levels of sarco(endo)plasmic reticulum Ca(2+)-ATPase, alpha(1)- and alpha(2)-subunits of Na(+)-K(+)-ATPase, phospholamban, ryanodine re...
متن کاملInduced overexpression of Na /Ca exchanger transgene: altered myocyte contractility, [Ca ]i transients, SR Ca contents, and action potential duration
Wang J, Chan TO, Zhang XQ, Gao E, Song J, Koch WJ, Feldman AM, Cheung JY. Induced overexpression of Na /Ca exchanger transgene: altered myocyte contractility, [Ca ]i transients, SR Ca contents, and action potential duration. Am J Physiol Heart Circ Physiol 297: H590–H601, 2009. First published June 12, 2009; doi:10.1152/ajpheart.00190.2009.—We have produced mice in which expression of the rat c...
متن کاملEffects of Overexpression of the Na-Ca Exchanger on [Ca]i Transients in Murine Ventricular Myocytes
We measured [Ca]i and [Na ]i in isolated transgenic (TG) mouse myocytes overexpressing the Na -Ca exchanger and in wild-type (WT) myocytes. In TG myocytes, the peak systolic level and amplitude of electrically stimulated (ES) [Ca]i transients (0.25 Hz) were not significantly different from those in WT myocytes, but the time to peak [Ca]i was significantly prolonged. The decline of ES [Ca]i tran...
متن کاملAltered contractility and [Ca2+]i homeostasis in phospholemman-deficient murine myocytes: role of Na+/Ca2+ exchange.
Phospholemman (PLM) regulates contractility and Ca(2+) homeostasis in cardiac myocytes. We characterized excitation-contraction coupling in myocytes isolated from PLM-deficient mice backbred to a pure congenic C57BL/6 background. Cell length, cell width, and whole cell capacitance were not different between wild-type and PLM-null myocytes. Compared with wild-type myocytes, Western blots indicat...
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